Glycosyl compounds
Chem-Impex International, Inc. Indoxyl b-D-glucoside | MFCD00047169 | 1G
Indoxyl b-D-glucoside, MFCD00047169, 1G
Chem-Impex International, Inc. n-Dodecyl-b-D-maltoside | 69227-93-6 | MFCD00043012 | 1G
n-Dodecyl-b-D-maltoside, 69227-93-6, MFCD00043012, 1G
Chem-Impex International, Inc. 5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside | 7240-90-6 | MFCD00005666 | 1G
5-Bromo-4-chloro-3-indolyl-b-D-galactopyranoside, 7240-90-6, MFCD00005666, 1G
Sigma Aldrich Fine Chemicals Biosciences Methocel A4M viscosity 3000-5500 mPa.s, 2 % in H2O(20 °C) | 9004-67-5 | MFCD00081763 | 100g
Methocel A4M viscosity 3000-5500 mPa.s, 2 % in H2O(20 °C) | 9004-67-5 | MFCD00081763 | 100g
![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 250 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-0.25.JPG-250.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 250 Milligrams
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
Research Products International Corp 4-Methylumbelliferyl-B-D-Xylopyranoside, 250 Milligrams
Fluorescent substrate for β-D-Xylosidase.
4-Methylumbelliferone: <50ppm
![Research Products International Corp [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 1 Gram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-N81000-1.02.JPG-250.jpg)
Research Products International Corp [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 1 Gram
Colorimetric and spectrophotometric substrate for detection of β-D-Galactosidase.
![Research Products International Corp Isopropyl-B-D-thio-galactopyranoside [IPTG], 100 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-I56000-100.021.JPG-250.jpg)
Research Products International Corp Isopropyl-B-D-thio-galactopyranoside [IPTG], 100 Grams
IPTG is combined with X-GAL to detect lac+ colonies or cells in a colorimetric assay. Distinguishes recombinants (white) from non- recombinants (blue) in cloning strategies using vectors such as Lambda-11, M13mp 18 and 19, pUC18 and 19, pUR222 containing the lacZ gene.
Dioxane-Free
Water Content: <0.49%
Research Products International Corp n-Dodecyl-B-D-maltoside, 5 Grams
Non-ionic detergent used in membrane protein studies. Reported to increase thermal stability of lipid-free rhodopsin in solution.
Sigma Aldrich Fine Chemicals Biosciences 4-Methylumbelliferyl Beta-D-Cellobioside | 72626-61-0 | MFCD00063279 | 100mg
4-Methylumbelliferyl Beta-D-Cellobioside | Mol Wt: 500.45 | 72626-61-0 | MFCD00063279 | 100mg
Sigma Aldrich Fine Chemicals Biosciences Methyl cellulose, 9004-67-5, MFCD00081763, 100 g
Viscosity: 4,000 cP, Methyl cellulose has been used: in colony forming assays, for sperm motility assessment, for the generation of spheroids, and in preparation of agar beads.
Research Products International Corp 4-Nitrophenyl-a-L-fucopyranoside, 1 Gram
Chromogenic substrate for detecting a-L-Fucosidase.
Research Products International Corp 2-Nitrophenyl-B-D-Xylopyranoside, 500 Milligrams
Spectrophotometric substrate for detection of β-Xylosidase.
Protect from moisture.
![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 1 Gram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-1.0NEW.JPG-250.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 1 Gram
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
AdipoGen n-Octyl-beta-D-glucopyranoside
Chemical. CAS 29836-26-8. Formula C14H28O6. MW 292.4. Non-ionic detergent for the functional solubilization, isolation, purification and crystallograpy of membrane proteins. Mild, non-denaturing detergent that is used for the solubilization and reconstitution of membrane-bound proteins in their native state and for the preparation of lipid vesicles. Its well-defined chemical structure, small uniform micelles and high water solubility make it superior to most other non-ionic detergents for membrane solubilization. The high critical micelle concentration 0.7% of n-octylglucoside facilitates ready removal from final protein extracts by dialysis or gel filtration. Used in lysis and solubilisation buffers for bacterial lipoprotein preparations, mitochondrial extracts or for determination of protein content by BCA assay. Can be used in 2D electrophoresis.